準(zhǔn)備和存儲
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
BD 553141 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block) 純化大鼠抗小
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Brand:BD Pharmingen?
Alternative Name:FcγRIII/FcγRII; Fcgr3/Fcgr2
Reactivity:Mouse (QC Testing)
Isotype:Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
Immunogen:Mouse BALB/c Macrophage J774
Application:Blocking, Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunoprecipitation (Reported)
Concentration:0.5 mg/ml
RRID:AB_394656
Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status:RUO
To specifically stain cells bearing FcγII and FcγIII receptors for flow cytometric analysis: Incubate cell suspension with this antibody (≤ 1 μg/million cells) followed by an appropriate fluorochrome-conjugated second-step reagent. To reduce Fc receptor-mediated binding by antibodies of interest or Fc receptor-mediated binding by PE-CY5 tandem dye conjugates to FcγII and FcγIII receptor-bearing mouse cells for flow cytometric analysis: 1. Preincubate cell suspension with Mouse BD Fc Block? purified anti-mouse CD16/CD32 mAb 2.4G2 (eg, ≤ 1 μg/million cells in 100 μl) at 4?C for 5 minutes. 2. Add antibody of interest directly to preincubated cells in the presence of Mouse BD Fc Block? (ie, Mouse BD Fc Block? need not be washed off before staining cells). 3. If anti-Ig second-step is necessary, a reagent must be chosen which will not bind to Mouse BD Fc Block? (eg, rat IgG2b, κ). For additional information on using Mouse BD Fc Block?, refer to our website protocol at http://www.bdbiosciences.com/pharmingen/protocols/Immunophenotyping.shtml推薦的實驗流程
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