The FITC Human Lineage Cocktail 4 has been designed to react with cells from the major hematopoietic lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, NK cells, erythrocytes, and granulocytes. This pre-diluted cocktail of twelve FITC-conjugated antibodies is designed to label lineage marker-positive cells for exclusion to facilitate the flow cytometric identification of lineage marker-negative hematopoietic stem cells in human cord blood and bone marrow. Components include clone RPA-2.10, which recognizes CD2; HIT3a, which recognizes CD3; RPA-T4, which recognizes CD4; M-T701, which recognizes CD7 ; HIT8a, which recognizes CD8; HI10a, which recognizes CD10; ICRF44, which recognizes CD11b; M5E2, which recognizes CD14; SJ25-C1, which recognizes CD19; 2H7, which recognizes CD20; B159, which recognizes CD56 and GA-R2 (HIR2), which recognizes CD235a. Additional fluorochrome-labeled reagents may be combined with the FITC Human Lineage Cocktail 4, such as CD34, CD38, CD90 and CD45RA conjugates, to characterize hematopoietic stem cell populations from cord blood or bone marrow.